Journal: bioRxiv

Article Title: Bacterial growth, communication and guided chemotaxis in 3D bioprinted hydrogel environments

doi: 10.1101/2021.08.10.455848

Figure Lengend Snippet: (A) Schematic image of a custom-built dual extrusion gel printer used for bacteria printing. Two syringe pumps were hydraulically connected to a print head holding two syringes, which were filled with bacteria-ink. The print heads were heated to 42°C, and the gel quickly solidified upon cooling on the base plate. (B) Bacteria ( E . coli DH5α) expressing either mRFP, mVenus, or mTurquoise were printed sequentially into hydrogel layers and incubated over night to represent the painting “Blue Fox” by the Munich expressionist Franz Marc. The image demonstrates the high precision of bioink deposition and the reproducibility of printing at specific locations. C) Using dual extrusion with blank and bacteria-ink, three-dimensional gel objects were printed that contained single layers of bacteria. Images of the 3D structures (top and side view) were taken using a Canon 80D camera, fluorescence images were obtained with a Nikon Ti2-E microscope from the bottom and from slices of the structures.

Article Snippet: As drying of the hydrogel leads to cell death, drying and consecutive shrinking was prevented using custom 3D printed plastic chambers (polylactic acid, BASF, Germany) and glass cover slides (round 18mm diameter and square 15mm, #1.5, Carl Roth, Germany) that are attached to the cover slides with instant glue.

Techniques: Bacteria, Expressing, Incubation, Fluorescence, Microscopy

Journal: eLife

Article Title: Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages

doi: 10.7554/eLife.55964

Figure Lengend Snippet: Parts for Headfixing System.

Article Snippet: 3D Printed Parts (Protolabs Watershed plastic) , , , , , , .

Techniques: Imaging